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Copper delivery through the skin has many potential applications and thus is widely studied, including the evaluation of the penetration ability of copper peptides 30 , biocompatibility of copper containing IUD 1 , the enhancement of skin absorption through copper-containing nanoparticles 10 and microneedle-mediated delivery 5

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After 2448 h, the cells were incubated with 100 nM TRaQ-G ligand for 1 h