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Consult with the treating healthcare provider regarding any patient-specific contraindications

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With cell lines of overexpression, the binding affinity and specificity were verified after aptamers with proper modification were produced

Supernatant was transferred to 1.5 mL microcentrifuge tube after which the remining pellet was resuspended in 400 L 20 C 40:40:20 Acetonitrile (ACN): MeOH: H2O extraction solvent and incubated on ice for 5 minutes