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The primers used were NLRP3 (5-ATGAGTGCTGCTTCGACATC-3, 5-TTGTCACTCAGGTCCAGCTC-3), and GAPDH (5-ATCATCCCTGCCTCTACTGC-3, 5-GTCAGGTCCACCACTGACAC-3)
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The nicotine present in cigarettes causes blood vessels to constrict, leading to reduced blood flow and oxygen supply to tissues
For instance, e-cigarette use has been linked to increased health risks, including increase thrombosis risk [203] as well as throat and mouth irritation, respiratory tract irritation, and behavioral changes among others [153]
As a result, smokers are more prone to skin infections, slower recovery from warts, cold sores, and fungal rashes, and a higher risk of more serious systemic infections.7
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