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Immunohistochemistry Immediately following acute electronic vape exposure (PG/VG N = 8, Nic N = 8) or the last session of repeated electronic vapor exposure (PG/VG N = 9, Nic N = 9), mice were anesthetized with pentobarbital (150 mg/kg, i.p.) and perfused with 1 phosphate-buffered saline (PBS) followed by 4% paraformaldehyde (PFA) in PBS

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The activities of ACC and HMG-CoA reductase were both inhibited by their phosphorylation, so the acceleration of lipid catabolism and cholesterol synthesis were performed