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if a vial does accidentally freeze, it should be discarded

If true, such a scenario would pose a formidable challenge to the research community, because distinguishing a physiological form of an otherwise normal endogenous brain protein from a pathologically misfolded/aggregated conformer is not trivial

Allergic reactions (rash, itching, swelling)

Most nasal spray protocols settle on 500mcg administered 2-3 times daily, totaling 1000-1500mcg per day

Therefore, the first place you need to look when the benefits of a methyl-B12 shot seems to wear off too soon is to retry the shots at the same dose and frequency but make the angle of attack much more severe, much closer to the horizontal plane, just under the skin