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Feeding the copper chelator bis-cyclohexanone-oxalyldihydrazone (cuprizone, CTZ) reduces the myelin sheath and activates microglial and astroglial cells in the CNS, providing a reproducible and reversible model of pathologic processes underlying white and gray matter demyelination [296,297,298,299,300,301]

Metabolite extraction and UPLC-MS analysis The samples were extracted using a pre-cooled methanol-water solution containing an internal standard

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Ongoing oxidative stress is linked to many chronic diseases (1) including cancer, cardiovascular disease, diabetes, osteoarthritis, and dementia