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matK was amplified with AccuStart II PCR SuperMix (QuantaBio #89235-018) and primers 5CGTACAGTACTTTTGTGTTTACGAG3 and 5ACCCAGTCCATCTGGAAATCTTGGTTC3 (250 nM final concentration) [50] using the following program: 3 min at 95 C, 40 (30 s at 95 C, 40 s at 60 C, 60 s at 72 C), and 5 min