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The medium was replaced, and after 8 h the cell supernatant was collected and co-incubated with RAW264.7 cells for 4 h

Materials and methods Systematic review The Literature review covered January 2002 to May 2023 in the medical database (PubMed)

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Tumor-draining lymph nodes (TDLNs) and tumor tissues from mice upon various treatments were collected to study the changes in relevant immune indicators