(5) The released oocytes, including cumulus oocyte complexes (COC) and denuded oocytes (DO) in the germinal vehicle (GV) stage were collected by aspirator tubes (Sigma-Aldrich), and transferred into another drop of IBMX-M2 media overlayed with paraffin oil (EMD Millipore Corporation, USA), which was pre-warmed to 37 C
High-dose B12 supplements, even 1,000 mcg daily, are generally safe because the body uses what it needs and excretes the rest
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Metabolism The metabolic fate of Somatotropin was not studied