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A single molecular entity simultaneously activates both the GLP-1 receptor and the amylin receptor (calcitonin receptor complex), combining two complementary satiety and metabolic pathways: GLP-1 receptor activation : Glucose-dependent insulin secretion, glucagon suppression, delayed gastric emptying, and central appetite reduction through hypothalamic signaling Amylin receptor activation : Additional satiety signaling through the area postrema, complementary gastric emptying delay, and glucagon suppression through a distinct mechanism The unimolecular design means both receptor activations occur at a fixed ratio determined by the molecular structure

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Rubio Osornio MdC, Custodio Ramrez V, Caldern Gmez D, Paz Tres C, Carvajal Aguilera KG

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To assess both cell viability and luciferase activity post-drug treatment, the ONE-Glo + Tox Luciferase Reporter and Cell Viability Assay (Cat