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Measurement of the expression of proteins by SDS-PAGE/Western Blotting and immunofluorescence microscopy Proteins (50 g) from the different sub cellular fractions of control and treated cells were separated on 12% SDS-PAGE and electrophoretically transferred onto nitrocellulose paper by Western blotting using the standard procedures of Laemmli [27] and Towbin et al

Theories include alteration of bile acid metabolism, small intestinal bacterial overgrowth, effects on intestinal motility and interference by metformin on vitamin B12-intrinsic factor absorption in the terminal ileum

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