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If you had used 2 mL of water instead of 1.5, the concentration would be lower (2.5 mg/mL) and your highest dose would require 96 units, nearly the entire syringe

its blockade increases serotonin release Promotes neurogenesis : TREK-1 knockout mice show increased hippocampal neurogenesis, and PE-22-28 recapitulates this effect pharmacologically within 4 days of treatment Induces synaptogenesis : TREK-1 blockade promotes dendritic growth and synaptic connection formation PE-22-28 inhibits TREK-1 with an IC50 of 0.12 nM, making it approximately 300-500 times more potent than spadin

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Thus, understanding if Salmonella indeed senses this specific molecule and characterizing how Salmonella responds to 5OP might shed light on the functions of this molecule